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Effect of Substrate Concentration on Enzyme Kinetics




  1. What is the need of adding DNS solution to the enzyme substrate solution?
  2. What is the problem in determining rates at low substrate concentration?
  3. Draw the Mchelis Menton plot  and the Line Weaver Burk Plot using the data given below and find the Vmax and Km from the Plot.


4.  Calculate the Activity of an amylase enzyme  which is diluted 1:100 times with phosphate buffer and incubated for 10 minutes at 37 degree Celsius. Given are the amount of maltose [mg] = 5.05 and the volume of enzyme used for the assay as 0.5ml.

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Amrita University, India 2009 - 2015

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